Cloning

How Dolly was created
Part 1: An Improbable Goal Scientists hoped that cloning healthy, mature sheep, rather than just creating lambs from embryonic cells, could produce a highly specialized sheep with large quantities of proteins in its milk. The proteins are believed to help treat diseases such as emphysema, hemophylia and cystic fibrosis.	Part 2: The Perfect Timing For years, scientists could not synchronize the growth of the egg and the cell. If one was off-synch, abnormal chromosomes would soon transform in the nucleus and thereby kill off the embryo. Dr. Wilmut achieved near perfection in the timing by putting the cells into hybernation; of the 277 eggs they began with, 247 lived through the process. Timing the growth in other species, however, has proved to scientists that cloning mature animals is extremely difficult; in the case of mice, they've concluded it can't be accomplished.	Part 3: Jump-start from a Jolt Though it has become a standard procedure in cloning, scientists are not sure why an electric pulse sent to the two cells causes them to meld together and activates development in the egg. They are learning, however, the shock doesn't fully mimic the activation process of a sperm, which could explain why just 29 of the remaining 247 cells live longer than six days.	Part 4: Surrogate Motherhood Despite the fact that the newly formed embryo is transplanted into the uterus of another ewe, scientists believe Dolly is a nearly exact carbon-copy of her genetic originating mother. In theory, that means an almost exact look-alike of John F. Kennedy Jr. could be produced by taking cells from his skin, melding their nuclei with any woman's egg that has had its nucleus removed and then planting the embryo in a surrogate mother. She would not have to be Jackie Kennedy.	Part 5: A 6-Year- Old in 7 Months? Scientists are eagerly watching Dolly to see if she exhibits the characteristics of her mother, a 6-year-old sheep, or those of her own age, just 7 months. As animals and humans age, changes occur in their DNA - such as decreasing fertility and increasing susceptibility to cancer and other diseases. If she prematurely ages, clones of mature animals would be useless to the agriculture industry.

Is cloning "unnatural"?

Not at all - some organisms in nature only reproduce using cloning - not only bacteria and yeasts, but also larger organisms like some snails and shrimp. Because in nature sexual reproduction is the only way to improve the genetic stock of a species, most asexual species tend to die off, but at least one - a shrimp called Artemia perthenogenetica - has survived for at least 30 million years. Many more species, including the aphid, reproduce by cloning most of the time, only reproducing sexually every few generations. Perhaps one day humankind may follow their lead.

Is an identical twin essentially the same as a clone?

Only if the clone is born at the same time from the same womb as its clone, as we now know that what a fetus is exposed to in the womb, in the way of nutrition or alcohol or drugs or perhaps even stress hormones, can influence its physical and mental development.

Could some lunatic clone Hitler if human cloning were perfected?

Just possibly - but they wouldn't get what they wanted. First, they would need some living cells from his body - unless it was frozen or otherwise preserved soon after death they would probably be unusable. More importantly, because of differences in the environment of the womb and upbringing clone Hitlers would not act, think or even necessarily look like the original.

Could clones be "farmed" to provide spare body parts for their "parent" clone without problems of tissue rejection?

Possibly, although we don't know enough yet to be confident that rejection would be eliminated entirely. You would also have to wait a number of years until the clone's organs were mature enough to transplant, and of course your actions would be highly illegal unless your clone was willing to act as a donor as a clone would be just as human as you or I. Even leaving aside the ethical concerns, with the progress that is being made in understanding and coping with tissue rejection, you would be more likely to have a pig's heart in your future than a clone's.

Would a clone have a soul?

Though we are not theologians if you grant souls to identical twins and to the various kinds of "test tube babies" already being born then it follows that a clone would have one too.

Could vital organs be grown using cloning without the rest of a body?

Possibly--but nobody is even close to knowing how. Contrary to scientists' expectations, the birth of Dolly shows it is possible to reprogram the cell of an adult (or at least its genome) so that it begins development all over again. This newly discovered flexibility means it may one day be possible to reprogram skin or blood cells so that they grow into "spare part" tissues and organs, rather than whole organisms. But the technical obstacles will be huge.

Could cloning be used to create "super warriors" or super-intelligent people?

Possibly - though we don't yet know enough about human genetics to do much "improving" of people. So far, because of ethical concerns, geneticists are concentrating on finding the causes of genetic diseases and then curing them. Cloning makes it easier to meddle with human and animal genes but is not necessarily genetic improvement, by itself. Even before recent discoveries a considerable amount of genetic improvement of animals was already taking place. A thoroughbred horse is essentially genetically engineered, for example.

Genetic engineering is rather a hit and miss technique. You try to add the gene you want in the right place, in the right cell, and sometimes that works. Before cloning, genetically engineering a sheep, for example, might have involved injecting DNA into the egg or early embryo. It was only once the animal grew up and was tested that it was possible to see if the desired genetic change had been introduced and stably incorporated into the animal's germ line.

Cloning, in theory, allows you to turn any cell into an animal. So instead of injecting DNA into an egg, you can shoot DNA into cells in a petri dish, allow them to grow and look among millions of cells for the type of genetic alteration you want. Since it is so much easier to manipulate cells than sheep - not to mention the fact that it is easier to feed, say, 100,000 cells than the same number of livestock - much rarer and more subtle gene manipulation can be accomplished, such as replacing one gene for another, or changing a single DNA letter of a gene.

Once you have cells with the desired genetic character, they are fused with an egg from which the chromosomes have been removed. Any animal that grows up from that experiment will have the genetic change in every cell of their body.

Could cloning be used to save endangered species?

At the moment its success rate is very low (Dolly was only cloned after 276 tries) but if this can be improved on it might well turn out to be useful to increase the population of hard-to-breed animals. Extinct animals (or animals without females) would be more difficult. A female can't normally give birth to an animal of a different species, but it is not yet clear whether a female of a closely-related species could give birth to a clone of a different species.